Composite

Part:BBa_K5143024:Design

Designed by: Perrine Ferdinand and Jess Philippon   Group: iGEM24_UnivLyon1-INSALyon   (2024-08-14)


Yellow protein and BioGlue protein secreted by Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1361
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1361
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1361
    Illegal BamHI site found at 1906
    Illegal BamHI site found at 2620
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1361
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1361
    Illegal PstI site found at 2789
    Illegal PstI site found at 3092
    Illegal PstI site found at 3185
    Illegal PstI site found at 3191
    Illegal NgoMIV site found at 1876
    Illegal AgeI site found at 1915
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

Ye L, Liu X, Li K, Li X, Zhu J, Yang S, Xu L, Yang M, Yan Y, Yan J. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials. Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127125. doi: 10.1016/j.ijbiomac.2023.127125. Epub 2023 Sep 28. PMID: 37776922.

References